On its fastest setting the attune can analyse ul per minute, with a maximum event rate of 35,000 events per second. We run both 96 and 384 well bead assays typically 1015 plex and the attune nxt is a real workhorse. The attune nxt is a very well built instrument, we are very happy with the attune nxt. Extensive research and science is included in the design to help improve functional outcomes for patients, performance for surgeons, and efficiency for. Attune nxt software, single license 5 licenses attune nxt. Used attune nxt flow cytometer certified genetool, inc. Invitrogen attune nxt gen autosampler a25529 new in box reuzeit.
Attune nxt acoustic focusing cytometer the invitrogen attune nxt acoustic focusing cytometer is a benchtop cytometer that uses acoustic pressure to confine the injected particles to a tight central line as the sample passes through the optical cell for interrogation. For questions about willmakers documents and interviews, see also willmaker faqs. Life tech attune nxt flow cytometer in ramsey, mn, usa. Attune nxt software is used to operate and maintain the attune nxt acoustic focusing cytometer it enables powerful user defined experimental analysis via an intuitive easy to use interface and provides advanced tools for both acquisition and analysis that are simple enough for users at all experience levels simplified use streamlined intuitive interface speed faster real time analysis. Autosampler technology with acousticassisted hydrodynamic focusing, the attune nxt flow cytometer figure 1 avoids compromise between data quality and higher sample rates by uncoupling cell alignment from sheath flow. Attunetm nxt acoustic focusing cytometer hardware user guide. D to understand, software user manuals are sometimes written from the point of view of a developer rather than a user. Power on the auto sampler before the attune nxt cytometer. As a result, the guide may make assumptions about th.
General electric ge appliances offers consumer home appliances. When we dont know whats going to happen, we tell ourselves stories about what might happen. The attune nxt has two lasers, 488 and 637 nm and can detect light in eight photomultiplier tubes pmts and one photodiode. Figure 3 demonstrates its ability to distinguish small particles, sometimes less than 0.
Attune nxt flow cytometer from thermo fisher scientific. The attune primary total knee system is designed with the goal of addressing the clinical needs of patients, surgeons, and hospital providers around the world. The attune nxt was designed for easeofuse, ideal for any environment where various levels of flow cytometry experience are found. Advanced tools are provided for both acquisition and analysis that are simple enough for users at all experience levels. Cf405m dylight 405 efluor 450 superbright 436 v450 512 25. Additional optimal fluorochrome options available on. The attune nxt flow cytometer was developed with the goal of removing barriers associated with flow cytometry and enabling new science. Presented plots and values are for illustrative purposes only and are based on customer reported. Particle size range the attune nxt cytometer is designed to detect particles with diameters from 0. For detail information about attune nxt, please visit the thermo fisher website. If you own a ge appliance, its important to have an owners manual to ensure proper maintenance and to answer any questions you may have. Fluorescence resolution the attune nxt cytometer is. Attune nxt acoustic focusing cytometer maintenance and troubleshooting guide catalog numbers a24858, a24859, a24860, a24861, a24862, a24863, a24864, 4473928 publication number 24234 revision b.
The thermofisher attune nxt analysis cytometer is equipped with four lasers having 16 parameter excitation. Bioplex 200 hardware manual,calibration, software and quick start manuals. Intelligent probeintelligent probe design minimizes clogging and carryover 1. The automated autosampler is a real plus when it is combined with the acoustic focusing because it allows you to count very diluted samples fast. Early rate through december 4 technology is part of a modern fascination wi. The attune nxt software enables user defined experimental analysis and the data can also be analysed using standard software including flowjo. Both lasers are flat top lasers with 50 m laser intercepts of the stream. Video which provides the viewer with easy to apply steps in the regular care and maintenance of the attune nxt. This growwithyou system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser.
Attune acoustic focusing cytometer thermo fisher bioz. Tables 1 and 2 list the common fluorochromes that the attune s lasers excite. Attune acoustic focusing cytometer minitower windows 7 64bit computer with 23inch flat panel monitor power cord with adapters usb cable user manual document cd attune nxt software quick reference card 1 vial of attune performance tracking beads 1 bottle of attune wash solution 1 bottle of attune 1x shutdown solution. Maintain the event rate at attune nxt instrument options. Financial support for the attune nxt was provided to maynooth university department of biology by science foundation ireland sfi under grant number 16ri3399. Data 1,500 1,000 cell counting spread 500 0 0 2 4 6 8 10 12 14 16 time 106 time vs. It uses sound waves which act like ripples in the stream, pushing all of the cells into the center, even when a large volume is being run. For safety alert words and symbols used in the user guide, see page 8. The attune nxt flow cytometer combines precision with performance in a true benchtop flow cytometer with up to 4 lasers and 16 parameters of detection.
Why the next great technology breakthrough shouldnt need a user manual. The blue 488 nm laser is 50 mw and the red 637 nm laser is 100 mw. Attune nxt software takes many of these manual tasks and fully automates them to help reduce time spent on instrument maintenance. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The attune nxt cytometer is designed to acquire data at up to 35,000 eventssecond. Heres your brains new user manual for uncertain times. The bandpass filters of the attune nxt are userexchangeable, easily slotted in and out of the optical bench depending on the users needs. Flow cytometry facility, centre for advanced technologies.
Attune nxt flow cytometer, blueredvioletyellow 1 each. Breathe easier with our openairways guide to better workouts, less coughing and wheezing, and just maybe a longer life. Commercial kits are available from several suppliers. The attune nxt flow cytometer can be equipped with up to 4 lasers and 16 detection channels. Analysis of intact viable cells was performed by gating the appropriate area and width of side and forward scatter to avoid cellular debris. Recommendations for accurate concentration measurements on.
The attune nxt software enables powerful userdefined experimental analysis via an intuitive, easytouse interface. Attune nxt acoustic focusing cytometer maintenance and. This includes bringing the power of flow cytometry to. According to the above needed specifications, either the attune nxt from life technologies or the lsr fortessa from bd could be purchased in our situation available. The attune nxt flow cytometer is a compact, benchtop cell analyzer that can be configured with up to 4 spatially separated lasers to flexibly design, run, and analyze multicolor panels of up to 14 colors. Catalog numbers a24858, a24859, a24860, a24861, a24862. Your toyota user manual provides important information for safe operation and routine maintenance for your car, truck or other equipment. What we like about the attune nxt in our lab, is the speed and effectiveness it provides for our cytokine and hormone beadbased assays.
The attune nxt flow cytometer, designed to use acousticassisted hydrodynamic focusing, and consequently achieves samplethroughput rates of of 12. Use this online manual answers basic questions about using quicken willmaker plus. Attune nxt flow cytometer with attune nxt autosample h. Recommendations for accurate concentration measurements. With sharp products in your home or office, you have the assurance of quality and innovation. The software provides an automated quality control instrument tracking system, important for collecting and analyzing accurate data requiring minimal user interaction.
Acousticassisted hydrodynamic focusing precisely aligns. This instrument is an attune nxt equipped with four lasers 488nm, 640nm, 405nm, and 561nm and is capable of detecting 16 parameters fsc, ssc and 14 fluorescent parameters. Broad compatibilitycompatible with many different plate formats, including 96well, 384well, and deepwell plates. The attune s acoustic focusing is designed to get around this problem. We combine it with our mplex software which reduces a complete plate of data in about a minute. How does the fluidics system of the attune nxt work. The invitrogen attune nxt flow cytometer combines precision with performance in a true benchtop flow cytometer with up to 4 lasers and 16 parameters of detection. Information in this document is subject to change without notice. Bottom row shows the same plots generated within kaluza after importing the fcs file. Often filled with jargon, acronyms, and directions that require a ph. Guidance for instrument start up and performance test using the attune nxt v3. Auto cleaningperforms automated cleaning when the instrument is. Invitrogen attune nxt gen autosampler a25529 new in box.
Attune nxt school of medicine clinical immunology and. Shop a large selection of flow cytometers products and learn more about attune nxt flow cytometer, blueredvioletyellow 1 each flow cytometers 1 each. Invitrogen attune nxt flow cytometer from thermo fisher. Volume 3jolene bradford, associate director flow cytometry, speaks about the software of the new attune nxt flow cyto. For a detailed description of the attune nxt software, refer to the attune nxt software user guide pub. Seven pmts detect fluorochromes and dyes, one pmt detects side scatter, and the photodiode detects forward scatter. Its very precise and gives reliable event counting. Attune nxt auto sampler catalog number 4473928 publication number 32905 revision a. Attune aligns with the forwardthinking and the selfmade label i give myself as an insurance agency of the 21st century. The attune nxt the next generation of flow cytometry.
Equipped with an autosampler compatible with many different plate formats, including 96well, 384well, and deepwell plates. Nowash, nolyse sample prep options with clogresistant fluidics. Extensive research and science is included in the design to help improve functional outcomes for patients, performance for. Attune nxt software, single license 5 licenses attune. Attune nxt flow cytometer, bluered 1 each flow cytometers. Life technologies attune nxt flow cytometry core facility.
Better workouts, less coughing and wheezing, even a longer life. Attune nxt flow cytometer thermofisher top row shows attune nxt data analyzed with system software. Maintain the event rate at attune nxt flow cytometer, designed using acousticassisted hydrodynamic focusing, achieves samplethroughput rates of of 12. Samples were subjected to facs using an attune acoustic focusing cytometer thermo fischer scientific equipped with a 488 nm and 637 nm laser and analyzed on the attune software v2. Sharp provides extensive user support to ensure that you know how to use the products you purchase. Here you will find software and related manuals for platts excel addin, platts on the net, and our data products. The attune nxt flow cytometer, designed using acousticassisted hydrodynamic focusing, achieves samplethroughput rates of of 12. For workflows and instructions on using the attune nxt acoustic focusing cytometer, refer to the attune nxt acoustic focusing cytometer user guide pub. The attune nxt at the core is equipped with an autosampler system to enable 96 well plate data acquisition. Financial support for the attune nxt was provided to maynooth university department of biology by science foundation ireland sfi. Attune nxt software time 45 minutes for 96well plate 60 minutes for 96well plate with 2 wash cycles 180 minutes for 384well plate using high throughput mode 240 minutes for 384well plate using standard mode, 2 wash cycles, carryover 0. For detailed maintenance and troubleshooting information, refer to the attune nxt maintenance and troubleshooting guide pub. Attune nxt flow cytometer thermo fisher scientific us. Attune insurance insurance as easy as it should be.
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